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Swimming pools of pre-developed primers might help generate comprehensive expression profiles, Particularly where assay sensitivity is critically significant.In contrast to random primers, oligo (dT) primers will not be suited to degraded samples but are excellent for developing cDNA libraries and advised to be used with reverse transcriptases (RTs

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It only signifies that the primers might amplify one or more other slice variants, As well as the just one you've got specified). Enabling this option will make it less difficult to discover gene-particular primers considering that there isn't a need to have to differentiate among splice variants. This option needs you to enter a refseq mRNA access

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